بررسی فعالیت آنتی اکسیدان‌ها و تنش اکسیداتیو ناشی از شوری در لاین‌های تریتیکاله و گندم در شرایط مزرعه

1 Department of agronomy and plant breeding, College of Agricultural, Isfahan University of Techchnology, Iran *Corresponding Author: Abstract: The effects of salt stress on the activity of ascorbate peroxidase (APX), glutathione reductase (GR), superoxide dismutase (SOD) enzymes, carotenoid content, the rate of lipid peroxidation (LP) in term of thiobarbituric acid-reactive substances (TBARS) content and grain yield were investigated. Eighteen triticale lines comprising 9 doubled haploid (DH) lines and 9 their corresponding F8 lines as well as two bread wheat cultivars ('Roshan' as a drought tolerant and 'Kavir' as a salt tolerant cultivar), were used. A randomized complete block design with three replications was used for each environmental condition (non-stressed and salt-stressed conditions) at the Research Farm of College of Agriculture, Isfahan University of Technology in a silty clay loam soil in 2008-2009. Both salt stressed and non-stressed experiments were irrigated with water having EC of 1 dS m-1 until mid-jointing stage (43 growth stage of Zadoks scale), and afterward salt-stressed experiment was irrigated with saline water containing 175 mM NaCl and EC= 16 dS m-1. Salinity led to an increase in the enzymes activities and LP and decrease in carotenoid content in the leaves of both triticale and wheat genotypes. An inverse and significant relationship between grain yield loss due to salinity stress with carotenoid content was observed under salinity stress (r = -0.54*). Under both conditions, negative and significant correlations (r = -0.61**) were observed between grain yield and LP. The orthogonal comparison between triticale line and wheat cultivars revealed the superiority of triticale lines for the production of antioxidants and less LP in triticale than wheat under both environmental conditions, which may be related to the development of relatively higher salt tolerant in triticale.